application of polymerase chain reaction in detection of egg drop syndrome (eds) virus

Authors

b. haerian

s.a. pourbakhsh

m. sehat

m. banani

s. shahsavandi

abstract

egg drop syndrome (eds) is caused by a hemagglutinating adenovirus which has become a major cause of lost egg production and sever economic losses in breeder and layer chicken flocks throughout the world. a pcr assay was optimized for detection of eds virus in inoculated allantoic fluids (afs) of duck and chicken emberyonated eggs. two strains of eds viruses were propagated in allantoic cavity of eggs. then the virus dna was extracted. pcr test was designed and carried out by specific primers. the 1900 bp band was detected in agar gel electrophoresis. serial two fold dilutions of infected allantoic fluid were prepared. the ha test and pcr assay were carried out for each dilution and finally results were compared to each other. the pcr assay could detect some negative ha titers. this study clearly indicates the superiority of pcr assay over ha test for detection of eds virus in afs.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Application of polymerase chain reaction in detection of egg drop syndrome (EDS) virus

  Egg drop syndrome (EDS) is caused by a hemagglutinating adenovirus which has become a major cause of lost egg production and sever economic losses in breeder and layer chicken flocks throughout the world. A PCR assay was optimized for detection of EDS virus in inoculated allantoic fluids (AFs) of duck and chicken emberyonated eggs. Two strains of EDS viruses were propagated in allantoic cavit...

full text

Detection of Varicella Zoster Virus (VZV) in the Benign and Malignant Breast Tumors by Polymerase chain Reaction

This study aimed to investigate the frequency of Varicella Zoster Virus (VZV) in benign and malignant breast tumors. A total of 24 carcinomas and 24 fibroadenomas paraffin embedded tumoral tissue samples were obtained from the pathology sections of Toos and Firoozgar hospitals in Tehran, Iran. All samples were collected from patients from June 2011 to February 2012. DNA was extracted from all s...

full text

COMPARISON OF CULTURE WITH POLYMERASE CHAIN REACTION FOR DETECTION OF UREAPLASMA UREALYTICUM IN ENDOCERVICAL SPECIMENS

Background: Ureaplasma urealyticum is known as a sexually transmitted agent, causing mainly urethritis, pelvic int1ammatory disease, spontaneous abortion, pyelonephritis, infertility, stillbirth, low birth weight neonatal meningititis, and neonatal pneumonia. U. urealyticum infections not only jeopardize fertility but also pose a risk for infertility treatment and resulting pregnancies. Dia...

full text

Detection of Leishmania DNA in paraffin embedded specimens of chronic lupoid leishmaniasis using polymerase chain reaction

Background: Chronic lupoid leishmaniasis (CLL) is a chronicform of cutaneous leishmaniasis that is usually resistant to antileishmania agents and leishmania is not seen in the histologicsections. We used polymerase chain reaction (PCR) to detectleishmania DNA in CLL specimens.Method: This descriptive cross sectional study was done on20 paraffin embedded specimens of CLL cases referred to theder...

full text

My Resources

Save resource for easier access later


Journal title:
archives of razi institute

Publisher: razi vaccine & serum research institute (rvsri)

ISSN 0365-3439

volume 62

issue 3 2016

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023